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Makorin-2 (MKRN2) is a highly conserved protein and yet its functions are largely unknown. We investigated the expression levels of  MKRN2  and  RAF1  in normal and malignant hematopoietic cells, and leukemia cell lines. We also attempted to delineate the role of  MKRN2  in umbilical cord blood CD34 +  stem/progenitor cells and K562 cell line by over-expression and inhibition of  MKRN2  through lentivirus transduction and shRNA nucleofection, respectively. Our results provided the first evidence on the ubiquitous expression of  MKRN2  in normal hematopoietic cells, embryonic stem cell lines, primary leukemia and leukemic cell lines of myeloid, lymphoid, erythroid and megakaryocytic lineages. The expression levels of  MKRN2  were generally higher in primary leukemia samples compared with those in age-matched normal BM cells. In all leukemia subtypes, there was no significant correlation between expression levels of  MKRN2  and  RAF1 . sh-MKRN2-silenced CD34 +  cells had a significantly lower proliferation capacity and decreased levels of the early stem/progenitor subpopulation (CFU-GEMM) compared with control cultures. Over-expression of  MKRN2  in K562 cells increased cell proliferation. Our results indicated possible roles of  MKRN2  in normal and malignant hematopoiesis.

Ubiquitous Expression of MAKORIN-2 in Normal and Malignant Hematopoietic Cells and Its Growth Promoting Activity