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Jasmonates (JAs) are important signaling molecules in plants and play crucial roles in stress responses, secondary metabolites' regulation, plant growth and development. In this study, the promoter of  AaAOC , which was the key gene of jasmonate biosynthetic pathway, had been cloned. GUS staining showed that  AaAOC  was expressed ubiquitiously in  A. annua .  AaAOC  gene was overexpressed under control of 35S promoter. RT-Q-PCR showed that the expression levels of  AaAOC  were increased from 1.6- to 5.2-fold in  AaAOC- overexpression transgenic  A. annua . The results of GC-MS showed that the content of endogenous jasmonic acid (JA) was 2- to 4.7-fold of the control level in  AaAOC -overexpression plants. HPLC showed that the contents of artemisinin, dihydroartemisinic acid and artemisinic acid were increased significantly in  AaAOC -overexpression plants. RT-Q-PCR showed that the expression levels of  FPS  (farnesyl diphosphate synthase),  CYP71AV1  (cytochrome P450 dependent hydroxylase) and  DBR2  (double bond reductase 2) were increased significantly in  AaAOC -overexpression plants. All data demonstrated that increased endogenous JA could significantly promote the biosynthesis of artemisinin in  AaAOC -overexpression transgenic  A.annua .

Overexpression of Allene Oxide Cyclase Improves the Biosynthesis of Artemisinin in Artemisia annua L.