English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We examined the properties of exotoxins produced by  Aeromonas trota  ( A. enteropelogenes ), one of the diarrheagenic species of  Aeromonadaceae . Nine of 19  A. trota  isolates that grew on solid media containing erythrocytes showed hemolytic activity. However, the hemolytic activities of the culture supernatants of these hemolytic strains of  A. trota  were markedly lower than those of  A. sobria  when cultured in liquid medium, and the amount of hemolysin detected by immunoblotting using antiserum against the hemolysin produced by  A. sobria  was also low. A mouse intestine loop assay using living bacterial cells showed that  A. trota  701 caused the significant accumulation of fluid, and antiserum against the hemolysin produced suppressed the enterotoxic action of  A. trota  701. These results indicated that  A. trota  701 was diarrheagenic and the hemolysin produced was the causative agent of the enterotoxic activity of  A. trota . The hemolysin in  A. sobria  was previously shown to be secreted in a preform (inactive form) and be activated when the carboxy-terminal domain was cleaved off by proteases in the culture supernatant. Since mature hemolysin was detected in the culture supernatants of  A. trota , we analyzed the extracellular protease produced by  A. trota . Fifteen of 19  A. trota  isolates that grew on solid media containing skim milk showed proteolytic activity. We subsequently found that most  A. trota  isolates possessed the serine protease gene, but not the metalloprotease gene. Therefore, we determined the nucleotide sequence of the serine protease gene and its chaperone  A. trota  gene. The results obtained revealed that the deduced amino acid sequences of serine protease and the chaperone were homologous to those of  A. sobria  with identities of 83.0% and 75.8%, respectively.

Properties of Hemolysin and Protease Produced by Aeromonas trota