English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Brassica juncea  Nonexpressor of pathogenesis-related genes 1 ( BjNPR1 ) has been introduced into pearl millet male fertility restorer line ICMP451 by  Agrobacterium tumefaciens- mediated genetic transformation. Transgenic pearl millet plants were regenerated from the phosphinothricin-resistant calli obtained after co-cultivation with  A. tumefaciens  strain LBA4404 harbouring Ti plasmid pSB111- bar - BjNPR1 . Molecular analyses confirmed the stable integration and expression of  BjNPR1  in transgenic pearl millet lines. Transgenes  BjNPR1  and  bar  were stably inherited and disclosed co-segregation in subsequent generations in a Mendelian fashion. Transgenic pearl millet hybrid ICMH451- BjNPR1  was developed by crossing male-sterile line 81A X homozygous transgenic line ICMP451- BjNPR1 . T 3  and T 4  homozygous lines of ICMP451- BjNPR1  and hybrid ICMH451- BjNPR1  exhibited resistance to three strains of downy mildew pathogen, while the untransformed ICMP451 and the isogenic hybrid ICMH451 plants were found susceptible. Following infection with  S. graminicola , differential expression of systemic acquired resistance pathway genes, UDP-glucose salicylic acid glucosyl transferase and pathogenesis related gene 1 was observed in transgenic ICMP451- BjNPR1  and untransformed plants indicating the activation of systemic acquired resistance pathway contributing to the transgene-mediated resistance against downy mildew. The transgenic pearl millet expressing  BjNPR1  showed resistance to multiple strains of  S. graminicola  and, as such, seems promising for the development of durable downy mildew resistant hybrids.

plos one

Transgenic Pearl Millet Male Fertility Restorer Line (ICMP451) and Hybrid (ICMH451) Expressing Brassica juncea Nonexpressor of Pathogenesis Related Genes 1 (BjNPR1) Exhibit Resistance to Downy Mildew Disease