English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

B-cell chronic lymphocytic leukemia (B-CLL) patients expressing unmutated immunoglobulin heavy variable regions ( IGHV s) use the  IGHV 1-69 B cell receptor (BCR) in 25% of cases. Since HIV-1 envelope gp41 antibodies also frequently use  IGHV 1-69 gene segments, we hypothesized that  IGHV 1-69 B-CLL precursors may contribute to the gp41 B cell response during HIV-1 infection. To test this hypothesis, we rescued 5  IGHV 1-69 unmutated antibodies as heterohybridoma IgM paraproteins and as recombinant IgG 1  antibodies from B-CLL patients, determined their antigenic specificities and analyzed BCR sequences.  IGHV 1-69 B-CLL antibodies were enriched for reactivity with HIV-1 envelope gp41, influenza, hepatitis C virus E2 protein and intestinal commensal bacteria. These  IGHV 1-69 B-CLL antibodies preferentially used  IGHD 3 and  IGHJ 6 gene segments and had long heavy chain complementary determining region 3s (HCDR3s) (≥21 aa).  IGHV 1-69 B-CLL BCRs exhibited a phenylalanine at position 54 (F 54 ) of the HCDR2 as do rare HIV-1 gp41 and influenza hemagglutinin stem neutralizing antibodies, while  IGHV 1-69 gp41 antibodies induced by HIV-1 infection predominantly used leucine (L 54 ) allelic variants. These results demonstrate that the B-CLL cell population is an expansion of members of the innate polyreactive B cell repertoire with reactivity to a number of infectious agent antigens including intestinal commensal bacteria. The B-CLL  IGHV 1-69 B cell usage of F 54  allelic variants strongly suggests that  IGHV 1-69 B-CLL gp41 antibodies derive from a restricted B cell pool that also produces rare HIV-1 gp41 and influenza hemagglutinin stem antibodies.

IGHV1-69 B Cell Chronic Lymphocytic Leukemia Antibodies Cross-React with HIV-1 and Hepatitis C Virus Antigens as Well as Intestinal Commensal Bacteria