English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Towards the goal of producing fully human polyclonal antibodies (hpAbs or hIgGs) in transchromosomic (Tc) cattle, we previously reported that Tc cattle carrying a human artificial chromosome (HAC) comprising the entire unrearranged human immunoglobulin (Ig) heavy-chain (h IGH ), kappa-chain (h IGK ), and lambda-chain (h IGL ) germline loci produced physiological levels of hIgGs when both of the bovine immunoglobulin mu heavy-chains, b IGHM  and b IGHML1 , were homozygously inactivated (b IGHM −/−  , b IGHML1 −/−  ; double knockouts or DKO). However, because endogenous bovine immunoglobulin light chain loci are still intact, the light chains are produced both from the h IGK  and h IGL  genomic loci on the HAC and from the endogenous bovine kappa-chain (b IGK ) and lambda-chain (b IGL ) genomic loci, resulting in the production of fully hIgGs (both Ig heavy-chains and light-chains are of human origin: hIgG/hIgκ or hIgG/hIgλ) and chimeric hIgGs (Ig heavy-chains are of human origin while the Ig light-chains are of bovine origin: hIgG/bIgκ or hIgG/bIgλ). To improve fully hIgG production in Tc cattle, we here report the deletion of the entire b IGL  joining (J) and constant (C) gene cluster (b IGLJ1-IGLC1  to b IGLJ5-IGLC5 ) by employing Cre/loxP mediated site-specific chromosome recombination and the production of triple knockout (b IGHM −/−  , b IGHML1 −/−   and b IGL −/−  ; TKO) Tc cattle. We further demonstrate that b IGL  cluster deletion greatly improves fully hIgGs production in the sera of TKO Tc cattle, with 51.3% fully hIgGs (hIgG/hIgκ plus hIgG/hIgλ).

plos one

Triple Immunoglobulin Gene Knockout Transchromosomic Cattle: Bovine Lambda Cluster Deletion and Its Effect on Fully Human Polyclonal Antibody Production