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Background  Although  Campylobacter jejuni  infections have a high prevalence worldwide and represent a significant socioeconomic burden, the underlying molecular mechanisms of induced intestinal immunopathology are still not well understood. We have recently generated a  C. jejuni  mutant strain NCTC11168:: cj0268c , which has been shown to be involved in cellular adhesion and invasion. The immunopathological impact of this gene, however, has not been investigated  in vivo  so far.    Methodology/Principal Findings  Gnotobiotic IL-10 deficient mice were generated by quintuple antibiotic treatment and perorally infected with  C. jejuni  mutant strain NCTC11168:: cj0268c , its complemented version (NCTC11168:: cj0268c -comp- cj0268c ), or the parental strain NCTC11168. Kinetic analyses of fecal pathogen loads until day 6 post infection (p.i.) revealed that knockout of  cj0268c  did not compromise intestinal  C. jejuni  colonization capacities. Whereas animals irrespective of the analysed  C. jejuni  strain developed similar clinical symptoms of campylobacteriosis (i.e. enteritis), mice infected with the NCTC11168:: cj0268c  mutant strain displayed significant longer small as well as large intestinal lengths indicative for less distinct  C. jejuni  induced pathology when compared to infected control groups at day 6 p.i. This was further supported by significantly lower apoptotic and T cell numbers in the colonic mucosa and lamina propria, which were paralleled by lower intestinal IFN-γ and IL-6 concentrations at day 6 following knockout mutant NCTC11168:: cj0268c  as compared to parental strain infection. Remarkably, less intestinal immunopathology was accompanied by lower IFN-γ secretion in  ex vivo  biopsies taken from mesenteric lymphnodes of NCTC11168:: cj0268c  infected mice versus controls.    Conclusion/Significance  We here for the first time show that the  cj0268c  gene is involved in mediating  C. jejuni  induced immunopathogenesis  in vivo . Future studies will provide further deep insights into the immunological and molecular interplays between  C. jejuni  and innate immunity in human campylobacteriosis.

plos one

Impact of Campylobacter jejuni cj0268c Knockout Mutation on Intestinal Colonization, Translocation, and Induction of Immunopathology in Gnotobiotic IL-10 Deficient Mice