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CDT-1 and CDT-2 are two cellodextrin transporters discovered in the filamentous fungus  Neurospora crassa . Previous studies focused on characterizing the role of these transporters in only a few conditions, including cellulose degradation, and the function of these two transporters is not yet completely understood. In this study, we show that deletion of  cdt-2 , but not  cdt-1 , results in growth defects not only on Avicel but also on xylan.  cdt-2  can be highly induced by xylan, and this mutant has a xylodextrin consumption defect. Transcriptomic analysis of the  cdt-2  deletion strain on Avicel and xylan showed that major cellulase and hemicellulase genes were significantly down-regulated in the  cdt-2  deletion strain and artificial over expression of  cdt-2  in  N. crassa  increased cellulase and hemicellulase production. Together, these data clearly show that CDT-2 plays a critical role in hemicellulose sensing and utilization. This is the first time a sugar transporter has been assigned a function in the hemicellulose degradation pathway. Furthermore, we found that the transcription factor XLR-1 is the major regulator of  cdt-2 , while  cdt-1  is primarily regulated by CLR-1. These results deepen our understanding of the functions of both cellodextrin transporters, particularly for CDT-2. Our study also provides novel insight into the mechanisms for hemicellulose sensing and utilization in  N. crassa , and may be applicable to other cellulolytic filamentous fungi.

Evidence of a Critical Role for Cellodextrin Transporte 2 (CDT-2) in Both Cellulose and Hemicellulose Degradation and Utilization in Neurospora crassa