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Campylobacter jejuni  is the leading cause of bacterial foodborne illness in the world, with symptoms ranging from acute diarrhea to severe neurological disorders. Contaminated poultry meat is a major source of  C. jejuni  infection, and therefore, strategies to reduce this organism in poultry, are expected to reduce the incidence of  Campylobacter -associated diseases. We have investigated whether oral administration of  C. jejuni -specific single-domain antibodies would reduce bacterial colonization levels in chickens. Llama single-domain antibodies specific for  C. jejuni  were isolated from a phage display library generated from the heavy chain IgG variable domain repertoire of a llama immunized with  C. jejuni  flagella. Two flagella-specific single-domain antibodies were pentamerized to yield high avidity antibodies capable of multivalent binding to the target antigen. When administered orally to  C. jejuni -infected two-day old chicks, the pentabodies significantly reduced  C. jejuni  colonization in the ceca.  In vitro , the motility of the bacteria was also reduced in the presence of the flagella-specific pentabodies, suggesting the mechanism of action is through either direct interference with flagellar motility or antibody-mediated aggregation. Fluorescent microscopy and Western blot analyses revealed specific binding of the anti-flagella pentabodies to the  C. jejuni  flagellin.

Pentavalent Single-Domain Antibodies Reduce Campylobacter jejuni Motility and Colonization in Chickens