English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The glassy-winged sharpshooter  Homalodisca vitripennis  (Hemiptera: Cicadellidae), is a xylem-feeding leafhopper and important vector of the bacterium  Xylella fastidiosa;  the causal agent of Pierce’s disease of grapevines. The functional complexity of the transcriptome of  H. vitripennis  has not been elucidated thus far. It is a necessary blueprint for an understanding of the development of  H. vitripennis  and for designing efficient biorational control strategies including those based on RNA interference.    Results  Here we elucidate and explore the transcriptome of adult  H. vitripennis  using high-throughput paired end deep sequencing and  de novo  assembly. A total of 32,803,656 paired-end reads were obtained with an average transcript length of 624 nucleotides. We assembled 32.9 Mb of the transcriptome of  H. vitripennis  that spanned across 47,265 loci and 52,708 transcripts. Comparison of our non-redundant database showed that 45% of the deduced proteins of  H. vitripennis  exhibit identity ( e -value ≤1 −5 ) with known proteins. We assigned Gene Ontology (GO) terms, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations, and potential Pfam domains to each transcript isoform. In order to gain insight into the molecular basis of key regulatory genes of  H. vitripennis , we characterized predicted proteins involved in the metabolism of juvenile hormone, and biogenesis of small RNAs (Dicer and Piwi sequences) from the transcriptomic sequences. Analysis of transposable element sequences of  H .  vitripennis  indicated that the genome is less expanded in comparison to many other insects with approximately 1% of the transcriptome carrying transposable elements.    Conclusions  Our data significantly enhance the molecular resources available for future study and control of this economically important hemipteran. This transcriptional information not only provides a more nuanced understanding of the underlying biological and physiological mechanisms that govern  H. vitripennis , but may also lead to the identification of novel targets for biorationally designed control strategies.

Sequencing and De Novo Assembly of the Transcriptome of the Glassy-Winged Sharpshooter (Homalodisca vitripennis)