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Background  Cats are definitive hosts of  Toxoplasma gondii  and play an essential role in the epidemiology of this parasite. The study aims at clarifying whether cats are able to develop specific antibodies against different clonal types of  T. gondii  and to determine by serotyping the  T. gondii  clonal types prevailing in cats as intermediate hosts in Germany.    Methodology  To establish a peptide-microarray serotyping test, we identified 24 suitable peptides using serological  T. gondii  positive (n=21) and negative cat sera (n=52). To determine the clonal type-specific antibody response of cats in Germany, 86 field sera from  T. gondii  seropositive naturally infected cats were tested. In addition, we analyzed the antibody response in cats experimentally infected with non-canonical  T. gondii  types (n=7).    Findings  Positive cat reference sera reacted predominantly with peptides harbouring amino acid sequences specific for the clonal  T. gondii  type the cats were infected with. When the array was applied to field sera from Germany, 98.8% (85/86) of naturally-infected cats recognized similar peptide patterns as  T. gondii  type II reference sera and showed the strongest reaction intensities with clonal type II-specific peptides. In addition, naturally infected cats recognized type II-specific peptides significantly more frequently than peptides of other type-specificities. Cats infected with non-canonical types showed the strongest reactivity with peptides presenting amino-acid sequences specific for both, type I and type III.    Conclusions  Cats are able to mount a clonal type-specific antibody response against  T. gondii . Serotyping revealed for most seropositive field sera patterns resembling those observed after clonal type II- T. gondii  infection. This finding is in accord with our previous results on the occurrence of  T. gondii  clonal types in oocysts shed by cats in Germany.

Serotyping of Toxoplasma gondii in Cats (Felis domesticus) Reveals Predominance of Type II Infections in Germany