English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Enterohemorrhagic Escherichia coli (EHEC) and atypical enteropathogenic  E. coli  (aEPEC) are important zoonotic pathogens that increasingly are becoming resistant to multiple antibiotics. Here we describe two plasmids, pO26-CRL 125  (125 kb) from a human O26:H- EHEC, and pO111-CRL 115  (115kb) from a bovine O111 aEPEC, that impart resistance to ampicillin, kanamycin, neomycin, streptomycin, sulfathiazole, trimethoprim and tetracycline and both contain atypical class 1 integrons with an identical IS 26 -mediated deletion in their 3´-conserved segment. Complete sequence analysis showed that pO26-CRL 125  and pO111-CRL 115  are essentially identical except for a 9.7 kb fragment, present in the backbone of pO26-CRL 125  but absent in pO111-CRL 115 , and several indels. The 9.7 kb fragment encodes IncI-associated genes involved in plasmid stability during conjugation, a putative transposase gene and three imperfect repeats. Contiguous sequence identical to regions within these pO26-CRL 125  imperfect repeats was identified in pO111-CRL 115  precisely where the 9.7 kb fragment is missing, suggesting it may be mobile. Sequences shared between the plasmids include a complete IncZ replicon, a unique toxin/antitoxin system, IncI stability and maintenance genes, a novel putative serine protease autotransporter, and an IncI1 transfer system including a unique shufflon. Both plasmids carry a derivate Tn 21  transposon with an atypical class 1 integron comprising a  dfrA5  gene cassette encoding resistance to trimethoprim, and 24 bp of the 3´-conserved segment followed by Tn 6026 , which encodes resistance to ampicillin, kanymycin, neomycin, streptomycin and sulfathiazole. The Tn 21 -derivative transposon is linked to a truncated Tn 1721 , encoding resistance to tetracycline, via a region containing the IncP-1α  oriV . Absence of the 5 bp direct repeats flanking Tn 3 -family transposons, indicates that homologous recombination events played a key role in the formation of this complex antibiotic resistance gene locus. Comparative sequence analysis of these closely related plasmids reveals aspects of plasmid evolution in pathogenic  E. coli  from different hosts.

plos one

Sequences of Two Related Multiple Antibiotic Resistance Virulence Plasmids Sharing a Unique IS26-Related Molecular Signature Isolated from Different Escherichia coli Pathotypes from Different Hosts