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Methylenetetrahydrofolate reductases (MTHFRs) play a key role in the biosynthesis of methionine in both prokaryotic and eukaryotic organisms. In this study, we report the identification of a novel T-DNA-tagged mutant WH672 in the rice blast fungus  Magnaporthe oryzae , which was defective in vegetative growth, conidiation and pathogenicity. Analysis of the mutation confirmed a single T-DNA insertion upstream of  MET13 , which encodes a 626-amino-acid protein encoding a MTHFR. Targeted gene deletion of  MET13  resulted in mutants that were non-pathogenic and significantly impaired in aerial growth and melanin pigmentation. All phenotypes associated with  Δmet13  mutants could be overcome by addition of exogenous methionine. The  M. oryzae  genome contains a second predicted MTHFR-encoding gene,  MET12 . The deduced amino acid sequences of Met13 and Met12 share 32% identity. Interestingly,  Δmet12  mutants produced significantly less conidia compared with the isogenic wild-type strain and grew very poorly in the absence of methionine, but were fully pathogenic. Deletion of both genes resulted in  Δmet13Δmet12  mutants that showed similar phenotypes to single  Δmet13  mutants. Taken together, we conclude that the MTHFR gene,  MET13 , is essential for infection-related morphogenesis by the rice blast fungus  M. oryzae .

The MET13 Methylenetetrahydrofolate Reductase Gene Is Essential for Infection-Related Morphogenesis in the Rice Blast Fungus Magnaporthe oryzae