English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We investigated the changing trend of various toxigenic  Clostridium difficile  isolates at a 3 500-bed hospital in Taiwan. Genetic relatedness and antimicrobial susceptibility of toxigenic  C. difficile  isolates were also examined.    Methods  A total of 110 non-repeat toxigenic  C. difficile  isolates from different patients were collected between 2002 and 2007. Characterization of the 110 toxigenic isolates was performed using agar dilution method, multilocus variable-number tandem-repeat analysis (MLVA) genotyping,  tcdC  genotyping, and toxinotyping.    Results  Among the 110 toxigenic isolates studied, 70 isolates harbored  tcdA  and  tcdB  (A + B + ) and 40 isolates harbored  tcdB  only (A − B + ). The annual number of A + B +  isolates considerably increased over the 6-year study ( P  = 0.055). A total of 109 different MLVA genotypes were identified, in which A + B +  isolates and A − B +  isolates were differentiated into two genetic clusters with similarity of 17.6%. Twenty-four (60%) of the 40 A − B +  isolates formed a major cluster, MLVA-group 1, with a similarity of 85%. Seven (6.4%) resistant isolates were identified, including two metronidazole-resistant and five vancomycin-resistant isolates.    Conclusions  This study indicated a persistence of a MLVA group 1 A − B +  isolates and an increase of A + B +  isolates with diverse MLVA types. Moreover,  C. difficile  isolates with antimicrobial resistance to metronidazole or vancomycin were found to have emerged. Continuous surveillance is warranted to understand the recent situation and control the further spread of the toxigenic  C. difficile  isolates, especially among hospitalized patients.

plos one

Molecular Epidemiology of Clostridium difficile at a Medical Center in Taiwan: Persistence of Genetically Clustering of A−B+ Isolates and Increase of A+B+ Isolates