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Piscirickettsia salmonis  is the bacterium that causes Piscirickettsiosis, a systemic disease of salmonid fish responsible for significant economic losses within the aquaculture industry worldwide. The growth of the bacterium for vaccine formulation has been traditionally accomplished by infecting eukaryotic cell lines, a process that involves high production costs and is time-consuming. Recent research has demonstrated that it is possible to culture pure  P. salmonis  in a blood containing (cell-free) medium.  In the present work we demonstrate the growth of  P. salmonis  in a liquid medium free from blood and serum components, thus establishing a novel and simplified bacteriological medium. Additionally, the new media reported provides improved growth conditions for  P. salmonis , where biomass concentrations of approximately 800 mg cell dry weight L −1  were obtained, about eight times higher than those reported for the blood containing medium. A 2- level full factorial design was employed to evaluate the significance of the main medium components on cell growth and an optimal temperature range of 23–27°C was determined for the microorganism to grow in the novel liquid media.  Therefore, these results represent a breakthrough regarding  P. salmonis  research in order to optimize pure  P. salmonis  growth in liquid blood and serum free medium.

A Novel Liquid Medium for the Efficient Growth of the Salmonid Pathogen Piscirickettsia salmonis and Optimization of Culture Conditions