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Coccidian parasites are of major importance in animal production, public health and food safety. The most frequently used representative in basic research on this group is   Toxoplasma gondii  . Although this parasite is well investigated there is no adequate  in vitro  model for its sexual development available and knowledge on this important life cycle phase is therefore scarce. The use ofIsosporasuis    , a sister taxon to   T. gondii   and the causative agent of piglet coccidiosis, could provide a solution for this. In the present study an  in vitro  model for neonatal porcine coccidiosis in cells representative for the  in vivo  situation in the piglet gut was developed and evaluated. The parasite development was investigated by light and transmission electron microscopy and optimum culture conditions were evaluated. Intestinal porcine epithelial cells (IPEC-J2) adequately representing the natural host cells supported the development of all endogenous life cycle stages ofI.  suis    , including gametocytes and oocysts. A concentration of 5% fetal calf serum in the culture medium led to highest gametocyte densities on day 12 post infection. Low infection doses (≤1 sporozoite for 100 host cells) were best for oocyst and gametocyte development. The presented system can also be used for immunostaining with established antibodies developed against   T. gondii   (in our case, anti- Tg IMC3 antibodies directed against the inner membrane complex 3). The complete life cycle ofI.  suisin a cell line representing the natural host cell type and species provides a unique model among coccidian parasites and can be used to address a wide range of topics, especially with regard to the sexual development of coccidia.

Isospora suis in an Epithelial Cell Culture System – An In Vitro Model for Sexual Development in Coccidia