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15-deoxy-Δ 12,14 -prostaglandin J 2  (15d-PGJ 2 ) is an anti-inflammatory downstream product of the cyclooxygenase enzymes. It has been implicated to play a protective role in a variety of inflammatory mediated diseases, including rheumatoid arthritis, neural damage, and myocardial infarctions. Here we show that 15d-PGJ 2  also plays a role in  Salmonella  infection.  Salmonella enterica  Typhimurium is a Gram-negative facultative intracellular pathogen that is able to survive and replicate inside phagocytic immune cells, allowing for bacterial dissemination to systemic sites.  Salmonella  species cause a wide range of morbidity and mortality due to gastroenteritis and typhoid fever. Previously we have shown that in mouse models of typhoid fever,  Salmonella  infection causes a major perturbation in the prostaglandin pathway. Specifically, we saw that 15d-PGJ 2  production was significantly increased in both liver and feces. In this work we show that 15d-PGJ 2  production is also significantly increased in macrophages infected with  Salmonella . Furthermore, we show that the addition of 15d-PGJ 2  to  Salmonella  infected RAW264.7, J774, and bone marrow derived macrophages is sufficient to significantly reduce bacterial colonization. We also show evidence that 15d-PGJ 2  is reducing bacterial uptake by macrophages. 15d-PGJ 2  reduces the inflammatory response of these infected macrophages, as evidenced by a reduction in the production of cytokines and reactive nitrogen species. The inflammatory response of the macrophage is important for full  Salmonella  virulence, as it can give the bacteria cues for virulence. The reduction in bacterial colonization is independent of the expression of  Salmonella  virulence genes SPI1 and SPI2, and is independent of the 15d-PGJ 2  ligand PPAR-γ. 15d-PGJ 2  also causes an increase in ERK1/2 phosphorylation in infected macrophages. In conclusion, we show here that 15d-PGJ 2  mediates the outcome of bacterial infection, a previously unidentified role for this prostaglandin.

15-Deoxy-Δ12,14-Prostaglandin J2 Inhibits Macrophage Colonization by Salmonella enterica Serovar Typhimurium