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During zebrafish development, a gradient of stromal-derived factor 1a (Sdf1a) provides the directional cue that guides the migration of the primordial germ cells (PGCs) to the gonadal tissue. Here we describe a method to produce large numbers of infertile fish by inducing ubiquitous expression of Sdf1a in zebrafish embryos resulting in disruption of the normal PGC migration pattern. A transgenic line of zebrafish,  Tg(hsp70:sdf1a-nanos3, EGFP) , was generated that expresses Sdf1a under the control of the heat-shock protein 70 ( hsp70 ) promoter and  nanos3  3?UTR. To better visualize the PGCs, the  Tg(hsp70:sdf1a-nanos3, EGFP)  fish were crossed with another transgenic line,  Tg(kop:DsRed-nanos3) , that expresses DsRed driven by the PGC-specific  kop  promoter. Heat treatment of the transgenic embryos caused an induction of Sdf1a expression throughout the embryo resulting in the disruption of their normal migration. Optimal embryo survival and disruption of PGC migration was achieved when transgenic embryos at the 4- to 8-cell stage were incubated at 34.5°C for 18 hours. Under these conditions, disruption of PGC migration was observed in 100% of the embryos. Sixty-four adult fish were developed from three separate batches of heat-treated embryos and all were found to be infertile males. When each male was paired with a wild-type female, only unfertilized eggs were produced and histological examination revealed that each of the adult male fish possessed severely under-developed gonads that lacked gametes. The results demonstrate that inducible Sdf1a expression is an efficient and reliable strategy to produce infertile fish. This approach makes it convenient to generate large numbers of infertile adult fish while also providing the capability to maintain a fertile brood stock.

Inducible Sterilization of Zebrafish by Disruption of Primordial Germ Cell Migration