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In mice, successful development and reproduction require that all cells, including germ cells, transition from a pluripotent to a differentiated state. This transition is associated with silencing of the pluripotency genes  Oct4  and  Nanog . Interestingly, these genes are repressed at different developmental timepoints in germ and somatic cells. Ovarian germ cells maintain their expression until about embryonic day (E) 14.5, whereas somatic cells silence them much earlier, at about E8.0. In both somatic cells and embryonic stem cells, silencing of  Oct4  and  Nanog  requires the nuclear receptor GCNF. However, expression of the  Gcnf  gene has not been investigated in fetal ovarian germ cells, and whether it is required for silencing  Oct4  and  Nanog  in that context is not known. Here we demonstrate that  Gcnf  is expressed in fetal ovarian germ cells, peaking at E14.5, when  Oct4  and  Nanog  are silenced. However, conditional ablation of the ligand-binding domain of  Gcnf  using a ubiquitous, tamoxifen-inducible Cre indicates that  Gcnf  is not required for the down-regulation of pluripotency genes in fetal ovarian germ cells, nor is it required for initiation of meiosis and oogenesis. These results suggest that the silencing of  Oct4  and  Nanog  in germ cells occurs via a different mechanism from that operating in somatic cells during gastrulation.

The Ligand Binding Domain of GCNF Is Not Required for Repression of Pluripotency Genes in Mouse Fetal Ovarian Germ Cells