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Huanglongbing (HLB) causes considerable economic losses to citrus industries worldwide. Its management depends on controlling of the Asian citrus Psyllid (ACP), the vector of the bacterium,  Candidatus  Liberibacter asiaticus ( C Las), the causal agent of HLB. Silencing genes by RNA interference (RNAi) is a promising tool to explore gene functions as well as control pests. In the current study, abnormal wing disc ( awd ) gene associated with wing development in insects is used to interfere with the flight of psyllids. Our study showed that transcription of  awd  is development-dependent and the highest level was found in the last instar (5 th ) of the nymphal stage. Micro-application (topical application) of dsRNA to 5 th  instar of nymphs caused significant nymphal mortality and adult wing-malformation. These adverse effects in ACP were positively correlated with the amounts of dsRNA used. A qRT-PCR analysis confirmed the dsRNA-mediated transcriptional down-regulation of the  awd  gene. Significant down-regulation was required to induce a wing-malformed phenotype. No effect was found when dsRNA- gfp  was used, indicating the specific effect of dsRNA -awd . Our findings suggest a role for  awd  in ACP wing development and metamorphosis.  awd  could serve as a potential target for insect management either via direct application of dsRNA or by producing transgenic plants expressing dsRNA -awd . These strategies will help to mitigate HLB by controlling ACP.

Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, Diaphorina citri Disrupts Adult Wing Development and Increases Nymph Mortality