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Background  Identification of pathogens associated with bovine mastitis is helpful in treatment and management decisions. However, such data from sub-Saharan Africa is scarce. Here we describe the distribution and antimicrobial susceptibility patterns of bacteria from cows with clinical mastitis in Kampala, Uganda. Due to high concern of zoonotic infections, isolates from milkmen are also described.    Methodology/Principal Findings  Ninety seven milk samples from cows with clinical mastitis and 31 nasal swabs from milkmen were collected (one sample per cow/human). Fifty eight (60%) Gram-positive isolates namely Staphylococci (21), Enterococci (16), Streptococci (13), Lactococci (5), Micrococci (2) and Arcanobacteria (1) were detected in cows; only one grew  Staphylococcus aureus . Furthermore, 24 (25%) coliforms namely  Escherichia coli  (12),  Klebsiella oxytoca  (5),  Proteus vulgaris  (2),  Serratia  (2),  Citrobacter  (1),  Cedecea  (1) and  Leclercia  (1) were identified. From humans, 24 Gram-positive bacteria grew, of which 11 were Staphylococci (35%) including four  Staphylococcus aureus . Upon susceptibility testing, methicillin-resistant coagulase-negative staphylococci (CoNS) were prevalent; 57%, 12/21 in cows and 64%, 7/11 in humans. However, methicillin-resistant  Staphylococcus aureus  was not detected. Furthermore, methicillin and vancomycin resistant CoNS were detected in cows ( Staphylococcus hominis ,  Staphylococcus lugdunensis ) and humans ( Staphylococcus scuiri ). Also, vancomycin and daptomycin resistant Enterococci ( Enterococcus faecalis  and  Enterococcus faecium,  respectively) were detected in cows. Coliforms were less resistant with three pan-susceptible isolates. However, multidrug resistant  Klebsiella ,  Proteus ,  Serratia, Cedecea , and  Citrobacter  were detected. Lastly, similar species grew from human and bovine samples but on genotyping, the isolates were found to be different. Interestingly, human and bovine  Staphylococcus aureus  were genetically similar (spa-CC435, spa-type t645 corresponding to ST121) but with different susceptibility patterns.    Conclusions/Significance  CoNS, Enterococci, Streptococci, and  Escherichia coli  are the predominant pathogens associated with clinical bovine-mastitis in Kampala, Uganda. Multidrug resistant bacteria are also prevalent. While similar species occurred in humans and cows, transmission was not detected.

Prevalence and Antimicrobial Susceptibility Patterns of Bacteria from Milkmen and Cows with Clinical Mastitis in and around Kampala, Uganda