English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Murine very small embryonic-like (VSEL) cells, defined by the Lin − Sca-1 + CD45 −  phenotype and small size, were described as pluripotent cells and proposed to be the most primitive hematopoietic precursors in adult bone marrow. Although their isolation and potential application rely entirely on flow cytometry, the immunophenotype of VSELs has not been extensively characterized. Our aim was to analyze the possible heterogeneity of Lin − Sca + CD45 −  population and investigate the extent to which VSELs characteristics may overlap with that of hematopoietic stem cells (HSCs) or endothelial progenitor cells (EPCs). The study evidenced that murine Lin − Sca-1 + CD45 −  population was heterogeneous in terms of c-Kit and KDR expression. Accordingly, the c-Kit + KDR − , c-Kit − KDR + , and c-Kit − KDR −  subpopulations could be distinguished, while c-Kit + KDR +  events were very rare. The c-Kit + KDR −  subset contained almost solely small cells, meeting the size criterion of VSELs, in contrast to relatively bigger c-Kit − KDR +  cells. The c-Kit − KDR − FSC low  subset was highly enriched in Annexin V-positive, apoptotic cells, hence omitted from further analysis. Importantly, using qRT-PCR, we evidenced lack of Oct-4A and Oct-4B mRNA expression either in whole adult murine bone marrow or in the sorted of Lin − Sca-1 + CD45 − FSC low  population, even by single-cell qRT-PCR. We also found that the Lin − Sca-1 + CD45 − c-Kit +  subset did not exhibit hematopoietic potential in a single cell-derived colony  in vitro  assay, although it comprised the Sca-1 + c-Kit + Lin −  (SKL) CD34 − CD45 − CD105 +  cells, expressing particular HSC markers. Co-culture of Lin − Sca-1 + CD45 − FSC low  with OP9 cells did not induce hematopoietic potential. Further investigation revealed that SKL CD45 − CD105 +  subset consisted of early apoptotic cells with fragmented chromatin, and could be contaminated with nuclei expelled from erythroblasts. Concluding, murine bone marrow Lin − Sca-1 + CD45 − FSC low  cells are heterogeneous population, which do not express the pluripotency marker Oct-4A. Despite expression of some hematopoietic markers by a Lin − Sca-1 + CD45 − c-Kit + KDR −  subset of VSELs, they do not display hematopoietic potential in a clonogenic assay and are enriched in early apoptotic cells.

Murine Bone Marrow Lin−Sca-1+CD45− Very Small Embryonic-Like (VSEL) Cells Are Heterogeneous Population Lacking Oct-4A Expression