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During mouse eye development, all retinal cell types are generated from the population of retina-committed progenitors originating from the neuroepithelium of the optic vesicle. Conditional gene inactivation provides an efficient tool for studying the genetic basis of the developing retina; however, the number of retina-specific Cre lines is limited. Here we report generation of the  mRx-Cre  BAC transgenic mouse line in which the expression of  Cre  recombinase is controlled by regulatory sequences of the mouse  Rx  gene, one of the earliest determinants of retinal development. When  mRx-Cre  transgenic mice were crossbred with the  ROSA26R  or  ROSA26R-EYFP  reporter lines, the Cre activity was observed in the optic sulcus from embryonic day 8.5 onwards and later in all progenitors residing in the neuroepithelium of the optic cup. Our results suggest that  mRx-Cre  provides a unique tool for functional genetic studies in very early stages of retinal development. Moreover, since eye organogenesis is dependent on the inductive signals between the optic vesicle and head surface ectoderm, the inductive ability of the optic vesicle can be analyzed using  mRx-Cre  transgenic mice.

Generation of mRx-Cre Transgenic Mouse Line for Efficient Conditional Gene Deletion in Early Retinal Progenitors