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An experimental model for immersion challenge of rainbow trout fry ( Oncorhynchus mykiss ) with  Flavobacterium psychrophilum , the causative agent of rainbow trout fry syndrome and bacterial cold water disease was established in the present study. Although injection-based infection models are reliable and produce high levels of mortality attempts to establish a reproducible immersion model have been less successful. Various concentrations of hydrogen peroxide (H 2 O 2 ) were evaluated before being used as a pre-treatment stressor prior to immersion exposure to  F. psychrophilum . H 2 O 2  accelerated the onset of mortality and increased mortality approximately two-fold; from 9.1% to 19.2% and from 14.7% to 30.3% in two separate experiments. Clinical signs observed in the infected fish corresponded to symptoms characteristically seen during natural outbreaks. These findings indicate that pre-treatment with H 2 O 2  can increase the level of mortality in rainbow trout fry after exposure to  F. psychrophilum .

Effect of Hydrogen Peroxide on Immersion Challenge of Rainbow Trout Fry with Flavobacterium psychrophilum