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We established an automated screening method to characterize adhesion of  Escherichia coli  to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic  E. coli  (EPEC). 104 intestinal  E. coli  isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93  E. coli  isolates from wild boars.  Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and  E. coli  genes or gene clusters. The gene  sfa/foc , encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however  E. coli  isolates from wild boars with the  sfa/foc  gene showed less adhesion and probiotic activity than  E. coli  with the  sfa/foc  gene isolated from domestic pigs after 6 hour incubation.  In conclusion, screening porcine  E. coli  for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between  E. coli  of domestic pigs and wild boars.

Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method