English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We aimed to obtain insights on the nature of a collection of isolates presumptively identified as atypical  Streptococcus pneumoniae  recovered from invasive and non-invasive infections in Spain. One-hundred and thirty-two isolates were characterized by: optochin susceptibility in ambient and CO 2 -enriched atmosphere; bile solubility; PCR-based assays targeting pneumococcal genes  lyt A,  ply ,  psp A,  cps A,  Spn 9802,  ali B-like ORF2, and a specific 16S rRNA region; multilocus sequence analysis; and antimicrobial susceptibility. By multilocus sequence analysis, 61 isolates were  S. pseudopneumoniae , 34 were pneumococci, 13 were  S. mitis , and 24 remained unclassified as non-pneumococci. Among  S. pseudopneumoniae  isolates, 51 (83.6%) were collected from respiratory tract samples; eight isolates were obtained from sterile sources. High frequency of non-susceptibility to penicillin (60.7%) and erythromycin (42.6%) was found. Only 50.8% of the  S. pseudopneumoniae  isolates displayed the typical optochin phenotype originally described for this species. None harbored the  cps A gene or the pneumococcal typical  lyt A restriction fragment length polymorphism. The  Spn 9802 and the specific 16S rRNA regions were detected among the majority of the  S. pseudopneumoniae  isolates (n = 59 and n = 49, respectively). The  ply  and  psp A genes were rarely found. A high genetic diversity was found and 59 profiles were identified. Among the  S. pneumoniae , 23 were capsulated and 11 were non-typeable. Three non-typeable isolates, associated to international non-capsulated lineages, were recovered from invasive disease sources. In conclusion, half of the atypical pneumococcal clinical isolates were, in fact,  S. pseudopneumoniae  and one-fourth were other streptococci. We identified  S. pseudopneumoniae  and non-typeable pneumococci as cause of disease in Spain including invasive disease.

Disease Isolates of Streptococcus pseudopneumoniae and Non-Typeable S. pneumoniae Presumptively Identified as Atypical S. pneumoniae in Spain