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Biofilm-forming cells are distinct from well characterized planktonic cells and aggregate in the extracellular matrix, the so-called extracellular polymeric substances (EPS). The  sinR  gene of  Bacillus subtilis  encodes a transcriptional regulator that is known to be involved in the biosynthesis of EPS in biofilms.  Porphyromonas gingivalis  inhabits the subgingival and extraradicular biofilm of humans and is one of the primary pathogens that cause progressive marginal and refractory apical periodontitis. Furthermore,  P. gingivalis  possesses PGN_0088, which encodes a putative ortholog of  B. subtilis sinR . Here, we investigated the role of PGN_0088 ( sinR ) on biofilm formation.  P. gingivalis  strains formed biofilms on saliva-coated glass surfaces in phosphate buffered saline. Quantitative analysis indicated that the biofilm of the  sinR  null mutant consisted of dense exopolysaccharide. Microscopic observations showed that the increased levels of exopolysaccharide produced by the  sinR  mutant changed the morphology of the EPS to a mesh-liked structure. Furthermore, physical analyses suggested that the enrichment of exopolysaccharide in the EPS enhanced the resistance of the biofilm to hydrodynamic shear force. The results presented here demonstrate  sinR  plays important roles in the ability of  P. gingivalis  strain ATCC 33277 to act as a negative mediator of exopolysaccharide accumulation and is indirectly associated with the structure of the EPS and the force of its adhesion to surfaces.

The sinR Ortholog PGN_0088 Encodes a Transcriptional Regulator That Inhibits Polysaccharide Synthesis in Porphyromonas gingivalis ATCC 33277 Biofilms