English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In paramutation, epigenetic information is transferred from one allele to another to create a gene expression state which is stably inherited over generations. Typically, paramutation describes a phenomenon where one allele of a gene down-regulates the expression of another allele. Paramutation has been described in several eukaryotes and is best understood in plants. Here we describe an unexpected paramutation-like  trans  SALK T-DNA interaction in Arabidopsis. Unlike most of the previously described paramutations, which led to gene silencing, the  trans  SALK T-DNA interaction caused an increase in the transcript levels of the endogenous gene ( COBRA ) where the T-DNA was inserted. This increased  COBRA  expression state was stably inherited for several generations and led to the partial suppression of the  cobra  phenotype. DNA methylation was implicated in this  trans  SALK T-DNA interaction since mutation of the DNA methyltransferase 1 in the suppressed  cobra  caused a reversal of the suppression. In addition, null mutants of the DNA demethylase  ROS1  caused a similar  COBRA  transcript increase in the  cobra  SALK T-DNA mutant as the  trans  T-DNA interaction. Our results provide a new example of a paramutation-like  trans  T-DNA interaction in Arabidopsis, and establish a convenient hypocotyl elongation assay to study this phenomenon. The results also alert to the possibility of unexpected endogenous transcript increase when two T-DNAs are combined in the same genetic background.

Paramutation-Like Interaction of T-DNA Loci in Arabidopsis