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Bacteria of the genus  Brucella  have the unusual capability to catabolize erythritol and this property has been associated with their virulence mainly because of the presence of erythritol in bovine foetal tissues and because the attenuated S19 vaccine strain is the only  Brucella  strain unable to oxydize erythritol. In this work we have analyzed the transcriptional changes produced in  Brucella  by erythritol by means of two high throughput approaches: RNA hybridization against a microarray containing most of  Brucella  ORF's constructed from the  Brucella  ORFeome and next generation sequencing of  Brucella  mRNA in an Illumina GAIIx platform. The results obtained showed the overexpression of a group of genes, many of them in a single cluster around the  ery  operon, able to co-ordinately mediate the transport and degradation of erythritol into three carbon atoms intermediates that will be then converted into fructose-6P (F6P) by gluconeogenesis. Other induced genes participating in the nonoxidative branch of the pentose phosphate shunt and the TCA may collaborate with the  ery  genes to conform an efficient degradation of sugars by this route. On the other hand, several routes of amino acid and nucleotide biosynthesis are up-regulated whilst amino acid transport and catabolism genes are down-regulated. These results corroborate previous descriptions indicating that in the presence of erythritol, this sugar was used preferentially over other compounds and provides a neat explanation of the the reported stimulation of growth induced by erythritol.

Evaluation of the Effects of Erythritol on Gene Expression in Brucella abortus