Open AccessGenerating Embryonic Stem Cells from the Inbred Mouse Strain DBA/2J, a Model of Glaucoma and Other Complex Diseases
Author(s) -
Laura G. Reinholdt,
Gareth R. Howell,
Anne Czechanski,
Danilo G. Macalinao,
Katharine H. MacNicoll,
ChyuanSheng Lin,
Leah Rae Donahue,
Simon W. M. John
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0050081
Subject(s) - embryonic stem cell , biology , stem cell , inbred strain , genetics , embryoid body , gene targeting , strain (injury) , blastocyst , microbiology and biotechnology , induced pluripotent stem cell , gene , embryogenesis , anatomy
Mouse embryonic stem (ES) cells are derived from the inner cell mass of blastocyst stage embryos and are used primarily for the creation of genetically engineered strains through gene targeting. While some inbred strains of mice are permissive to the derivation of embryonic stem cell lines and are therefore easily engineered, others are nonpermissive or recalcitrant. Genetic engineering of recalcitrant strain backgrounds requires gene targeting in a permissive background followed by extensive backcrossing of the engineered allele into the desired strain background. The inbred mouse strain DBA/2J is a recalcitrant strain that is used as a model of many human diseases, including glaucoma, deafness and schizophrenia. Here, we describe the generation of germ-line competent ES cell lines derived from DBA/2J mice. We also demonstrate the utility of DBA/2J ES cells with the creation of conditional knockout allele for Endothelin-2 ( Edn2 ) directly on the DBA/2J strain background.
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