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Background    Caenorhabditis elegans (C. elegans)  has become a widely used model to explore the effect of food constituents on health as well as on life-span extension. The results imply that besides essential nutrients several flavonoids are able to impact the aging process. What is less investigated is the bioavailability and biotransformation of these compounds in  C. elegans . In the present study, we focused on the soy isoflavone genistein and its metabolism in the nematode as a basis for assessing whether this model system mimics the mammalian condition.    Principal Findings    C. elegans  was exposed to 100 µM genistein for 48 hours. The worm homogenate was extracted and analyzed by liquid chromatography (LC). 11 metabolites of genistein were detected and characterized using LC electrospray ionization mass spectrometry. All genistein metabolites formed by  C. elegans  were found to be sugar conjugates, primarily genistein- O -glucosides. The dominant metabolite was identified as genistein-7- O -phosphoglucoside. Further interesting metabolites include two genistein-di- O -glycosides, a genistein- O -disaccharide as well as a genistein- O -phosphodisaccharide.    Conclusions/Significance  Our study provides evidence for a novel biotransformation pathway in  C. elegans  leading to conjugative metabolites which are not known for mammals. The metabolism of genistein in mammals and in  C. elegans  differs widely which may greatly impact the bioactivity. These differences need to be appropriately taken into consideration when  C. elegans  is used as a model to assess possible health or aging effects.

Formation of Phosphoglycosides in Caenorhabditis elegans: A Novel Biotransformation Pathway