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Background  MicroRNAs (miRNAs) are a class of small RNAs that are implicated in the control of eukaryotic gene expression by binding to the 3′UTR of target mRNAs. Several algorithms have been developed for miRNA target prediction however, experimental validation is still essential for the correct identification of miRNA targets. We have recently predicted that Neuropilin2a ( Nrp2a ), a vascular endothelial growth factor receptor which is essential for normal developmental angiogenesis in zebrafish, is a dre-miR-2188 target.    Methodology  Here we show that dre-miR-2188 targets the 3′-untranslated region (3′UTR) of  Nrp2a  mRNA and is implicated in proper intersegmental vessel development  in vivo . Over expression of miR-2188 in zebrafish embryos down regulates  Nrp2a  expression and results in intersegmental vessel disruption, while its silencing increases  Nrp2a  expression and intersegmental vessel sprouting. An  in vivo  GFP sensor assay based on a fusion between the GFP coding region and the  Nrp2a  3′UTR confirms that miR-2188 binds to the 3′UTR of  Nrp2a  and inhibits protein translation.    Conclusions  We demonstrate that miR-2188 targets  Nrp2a  and affects intersegmental vessel development in zebrafish embryos.

Dre-miR-2188 Targets Nrp2a and Mediates Proper Intersegmental Vessel Development in Zebrafish Embryos