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Hyaluronan is synthesized within the cytoplasm and exported into the extracellular matrix through the cell membrane of fibroblasts by the MRP5 transporter. In order to meet the law of electroneutrality, a cation is required to neutralize the emerging negative hyaluronan charges. As we previously observed an inhibiting of hyaluronan export by inhibitors of K +  channels, hyaluronan export was now analysed by simultaneously measuring membrane potential in the presence of drugs. This was done by both hyaluronan import into inside-out vesicles and by inhibition with antisense siRNA. Hyaluronan export from fibroblast was particularly inhibited by glibenclamide, ropivacain and BaCl 2  which all belong to ATP-sensitive inwardly-rectifying K ir  channel inhibitors. Import of hyaluronan into vesicles was activated by 150 mM KCl and this activation was abolished by ATP. siRNA for the K +  channels K ir 3.4 and K ir 6.2 inhibited hyaluronan export. Collectively, these results indicated that hyaluronan export depends on concurrent K +  efflux.

Hyaluronan Export through Plasma Membranes Depends on Concurrent K+ Efflux by Kir Channels