English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Fibroblast growth factor 10 (Fgf10) is a key regulator of diverse organogenetic programs during mouse development, particularly branching morphogenesis.  Fgf10 -null mice suffer from lung and limb agenesis as well as cecal and colonic atresia and are thus not viable. To date, the  Mlcv1v-nLacZ-24  transgenic mouse strain (referred to as  Fgf10 LacZ  ), which carries a  LacZ  insertion 114 kb upstream of exon 1 of  Fgf10  gene, has been the only strain to allow transient lineage tracing of  Fgf10 -positive cells. Here, we describe a novel  Fgf10 Cre-ERT2   knock-in line ( Fgf10 iCre  ) in which a Cre-ERT2-IRES-YFP cassette has been introduced in frame with the ATG of exon 1 of  Fgf10  gene. Our studies show that Cre-ERT2 insertion disrupts  Fgf10  function. However, administration of tamoxifen to  Fgf10 iCre  ;  Tomato flox   double transgenic embryos or adult mice results in specific labeling of  Fgf10 -positive cells, which can be lineage-traced temporally and spatially. Moreover, we show that the  Fgf10 iCre   line can be used for conditional gene inactivation in an inducible fashion during early developmental stages. We also provide evidence that transcription factors located in the first intron of  Fgf10  gene are critical for maintaining  Fgf10  expression over time. Thus, the  Fgf10 iCre   line should serve as a powerful tool to explore the functions of  Fgf10  in a controlled and stage-specific manner.

Characterization of a Novel Fibroblast Growth Factor 10 (Fgf10) Knock-In Mouse Line to Target Mesenchymal Progenitors during Embryonic Development