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Arginine methylation of non-histone proteins by protein arginine methyltransferase (PRMT) has been shown to be important for various biological processes from yeast to human. Although PRMT genes are well conserved in fungi, none of them have been functionally characterized in plant pathogenic ascomycetes. In this study, we identified and characterized all of the four predicted PRMT genes in  Fusarium graminearum , the causal agent of Fusarium head blight of wheat and barley. Whereas deletion of the other three PRMT genes had no obvious phenotypes, the Δ amt1  mutant had pleiotropic defects.  AMT1  is a predicted type I PRMT gene that is orthologous to  HMT1  in  Saccharomyces cerevisiae . The Δ amt1  mutant was slightly reduced in vegetative growth but normal in asexual and sexual reproduction. It had increased sensitivities to oxidative and membrane stresses. DON mycotoxin production and virulence on flowering wheat heads also were reduced in the Δ amt1  mutant. The introduction of the wild-type  AMT1  allele fully complemented the defects of the Δ amt1  mutant and Amt1-GFP fusion proteins mainly localized to the nucleus. Hrp1 and Nab2 are two hnRNPs in yeast that are methylated by Hmt1 for nuclear export. In  F. graminearum ,  AMT1  is required for the nuclear export of FgHrp1 but not FgNab2, indicating that yeast and  F. graminearum  differ in the methylation and nucleo-cytoplasmic transport of hnRNP components. Because  AMT2  also is a predicted type I PRMT with limited homology to yeast  HMT1 , we generated the Δ amt1  Δ amt2  double mutants. The Δ amt1  single and Δ amt1  Δ amt2  double mutants had similar defects in all the phenotypes assayed, including reduced vegetative growth and virulence. Overall, data from this systematic analysis of PRMT genes suggest that  AMT1 , like its ortholog in yeast, is the predominant PRMT gene in  F. graminearum  and plays a role in hyphal growth, stress responses, and plant infection.

The AMT1 Arginine Methyltransferase Gene Is Important for Plant Infection and Normal Hyphal Growth in Fusarium graminearum