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Mangotoxin is an antimetabolite toxin produced by certain  Pseudomonas syringae  pv. syringae strains. This toxin is an oligopeptide that inhibits ornithine N-acetyl transferase, a key enzyme in the biosynthesis of ornithine and arginine. Previous studies have reported the involvement of the putative nonribosomal peptide synthetase MgoA in virulence and mangotoxin production. In this study, we analyse a new chromosomal region of  P. syringae  pv. syringae UMAF0158, which contains six coding sequences arranged as an operon ( mbo  operon). The  mbo  operon was detected in only mangotoxin-producing strains, and it was shown to be essential for the biosynthesis of this toxin. Mutants in each of the six ORFs of the  mbo  operon were partially or completely impaired in the production of the toxin. In addition,  Pseudomonas  spp. mangotoxin non-producer strains transformed with the  mbo  operon gained the ability to produce mangotoxin, indicating that this operon contains all the genetic information necessary for mangotoxin biosynthesis. The generation of a single transcript for the  mbo  operon was confirmed and supported by the allocation of a unique promoter and Rho-independent terminator. The phylogenetic analysis of the  P. syringae  strains harbouring the  mbo  operon revealed that these strains clustered together.

The mbo Operon Is Specific and Essential for Biosynthesis of Mangotoxin in Pseudomonas syringae