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This investigation examines the influence of alpha-toxin (Hla) during USA300 infection of human leukocytes. Survival of an USA300 isogenic deletion mutant of  hla  (USA300Δ hla ) in human blood was comparable to the parental wild-type strain and polymorphonuclear leukocyte (PMN) plasma membrane permeability caused by USA300 did not require Hla. Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) following infection by USA300, USA300Δ hla , and USA300Δ hla  transformed with a plasmid over-expressing Hla (USA300Δ hla  Comp) demonstrated this toxin plays a significant role inducing plasma membrane permeability of CD14 + , CD3 + , and CD19 +  PBMCs. Rapid plasma membrane permeability independent of Hla was observed for PMNs, CD14 +  and CD19 +  PBMCs following intoxication with USA300 supernatant while the majority of CD3 +  PBMC plasma membrane permeability induced by USA300 required Hla. Addition of recombinant Hla to USA300Δ hla  supernatant rescued CD3 +  and CD19 +  PBMC plasma membrane permeability generated by USA300 supernatant. An observed delay in plasma membrane permeability caused by Hla in conjunction with Annexin V binding and ApoBrdU Tunel assays examining PBMCs intoxicated with recombinant Hla or infected with USA300, USA300Δ hla,  USA300Δ hla  Comp, and USA300Δ saeR/S  suggest Hla induces programmed cell death of monocytes, B cells, and T cells that results in plasma membrane permeability. Together these findings underscore the importance of Hla during  S. aureus  infection of human tissue and specifically demonstrate Hla activity during USA300 infection triggers programmed cell death of human monocytes, T cells and B cells that leads to plasma membrane permeability.