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BnAP2 , an  APETALA2  ( AP2 )-like gene, has been isolated from  Brassica napus  cultivar Zhongshuang 9. The cDNA of  BnAP2 , with 1, 299 bp in length, encoded a transcription factor comprising of 432 amino acid residues.  Results  from complementary experiment indicated that  BnAP2  was completely capable of restoring the phenotype of  Arabidopsis ap2-11  mutant. Together with the sequence and expression data, the complementation data suggested that  BnAP2  encodes the ortholog of  AtAP2 . To address the transcriptional activation of  BnAP2 , we performed transactivation assays in yeast. Fusion protein of BnAP2 with GAL4 DNA binding domain strongly activated transcription in yeast, and the transactivating activity of BnAP2 was localized to the N-terminal 100 amino acids. To further study the function of  BnAP2  involved in the phenotype of  B. napus , we used a transgenic approach that involved targeted RNA interference (RNAi) repression induced by ihp-RNA. Floral various phenotype defectives and reduced female fertility were observed in  B. napus BnAP2 -RNAi lines. Loss of the function of  BnAP2  gene also resulted in delayed sepal abscission and senescence with the ethylene-independent pathway. In the strong  BnAP2 -RNAi lines, seeds showed defects in shape, structure and development and larger size. Strong  BnAP2 -RNAi and wild-type seeds initially did not display a significant difference in morphology at 10 DAF, but the development of  BnAP2 -RNAi seeds was slower than that of wild type at 20 DAF, and further at 30 DAF, wild-type seeds were essentially at their final size, whereas  BnAP2 -RNAi seeds stopped growing and developing and gradually withered.

Functional Identification and Characterization of the Brassica Napus Transcription Factor Gene BnAP2, the Ortholog of Arabidopsis Thaliana APETALA2