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Current cyanobacterial model organisms were not selected for their growth traits or potential for the production of renewable biomass, biofuels, or other products. The cyanobacterium strain BL0902 emerged from a search for strains with superior growth traits. Morphology and 16S rRNA sequence placed strain BL0902 in the genus  Leptolyngbya .  Leptolyngbya  sp. strain BL0902 (hereafter  Leptolyngbya  BL0902) showed robust growth at temperatures from 22°C to 40°C and tolerated up to 0.5 M NaCl, 32 mM urea, high pH, and high solar irradiance. Its growth rate under outdoor conditions rivaled  Arthrospira  (“ pirulina”  strains.  Leptolyngbya  BL0902 accumulated higher lipid content and a higher proportion of monounsaturated fatty acids than  Arthrospira  strains. In addition to these desirable qualities,  Leptolyngbya  BL0902 is amenable to genetic engineering that is reliable, efficient, and stable. We demonstrated conjugal transfer from  Escherichia coli  of a plasmid based on RSF1010 and expression of spectinomycin/streptomycin resistance and  yemGFP  reporter transgenes. Conjugation efficiency was investigated in biparental and triparental matings with and without a “elper”plasmid that carries DNA methyltransferase genes, and with two different conjugal plasmids. We also showed that  Leptolyngbya  BL0902 is amenable to transposon mutagenesis with a Tn 5  derivative. To facilitate genetic manipulation of  Leptolyngbya  BL0902, a conjugal plasmid vector was engineered to carry a  trc  promoter upstream of a Gateway recombination cassette. These growth properties and genetic tools position  Leptolyngbya  BL0902 as a model cyanobacterial production strain.

Gene Transfer in Leptolyngbya sp. Strain BL0902, a Cyanobacterium Suitable for Production of Biomass and Bioproducts