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Background  In budding yeast, the highly conserved Tel2 protein is part of several complexes and its main function is now believed to be in the biogenesis of phosphatidyl inositol 3-kinase related kinases.    Principal Findings  To uncover potentially novel functions of Tel2, we set out to isolate temperature-sensitive (ts) mutant alleles of  TEL2  in order to perform genetic screenings.  MED15 / GAL11 , a subunit of Mediator, a general regulator of transcription, was isolated as a suppressor of these mutants. The isolated  tel2  mutants exhibited a short telomere phenotype that was partially rescued by  MED15 / GAL11  overexpression. The  tel2 - 15 mutant was markedly deficient in the transcription of  EST2 , coding for the catalytic subunit of telomerase, potentially explaining the short telomere phenotype of this mutant. In parallel, a two-hybrid screen identified an association between Tel2 and Rvb2, a highly conserved member of the AAA+ family of ATPases further found by  in vivo  co-immunoprecipitation to be tight and constitutive. Transiently overproduced Tel2 and Med15/Gal11 associated together, suggesting a potential role for Tel2 in transcription. Other Mediator subunits, as well as  SUA7 /TFIIB, also rescued the  tel2 - ts  mutants.    Significance  Altogether, the present data suggest the existence of a novel role for Tel2, namely in transcription, possibly in cooperation with Rvb2 and involving the existence of physical interactions with the Med15/Gal11 Mediator subunit.

Genetic and Physical Interactions between Tel2 and the Med15 Mediator Subunit in Saccharomyces cerevisiae