English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Several SNPs located in or around the  IL28B  gene are associated with response of patients infected with Hepatitis C virus to treatment with pegylated interferon-α +/− ribavirin or with spontaneous clearance of the virus. The results of such studies are so compelling that future treatment approaches are likely to involve clinical decisions being made on the basis of a patient's genotype. Since  IL28B  is a paralogue of  IL28A  with greater than 95% sequence identity, it is possible that without genotyping assay specificity, sequences in  IL28A  may contribute to genotype identification, and potentially confound treatment decisions. This study aimed to 1) examine DNA sequences in  IL28B  surrounding each of the reported associated SNPs and the corresponding regions in  IL28A ; and 2) develop a robust assay for rs12979860, the most ‘cosmopolitan’ SNP most strongly associated with treatment response across all global populations studied to date. Bioinformatic analysis of genomic regions surrounding  IL28A  and  IL28B  demonstrated that 3 SNPs were unique to  IL28B , whereas the remaining 6 SNP regions shared >93% identity between  IL28A  and  IL28B . Using a panel of DNA samples, PCR amplification followed by Sanger sequencing was used to examine  IL28B  SNPs and the corresponding regions in  IL28A . For the overlapping SNPs, all 6 in  IL28B  were confirmed to be polymorphic whereas the corresponding positions in  IL28A  were monomorphic. Based upon  IL28A  and  IL28B  sequence data, a specific TaqMan® assay was developed for SNP rs12979860 that was 100% concordant to the sequence-derived genotypes. Analysis using a commercial assay identified one discordant result which led to a change in their genotype-calling algorithm. Where future treatment decisions are made upon the results of genotyping assays, it is very important that results are concordant with data from a sequence-based format. This is especially so in situations where designing specific PCR primers is a challenge.

Sequence Analysis of the IL28A/IL28B Inverted Gene Duplication That Contains Polymorphisms Associated with Treatment Response in Hepatitis C Patients