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Due to drawbacks of live attenuated vaccines, much more attention has been focused on screening of  Brucella  protective antigens as subunit vaccine candidates.  Brucella  is a facultative intracellular bacterium and cell mediated immunity plays essential roles for protection against  Brucella  infection. Identification of  Brucella  antigens that present T-cell epitopes to the host could enable development of such vaccines. In this study, 45 proven or putative pathogenesis-associated factors of  Brucella  were selected according to currently available data. After expressed and purified, 35 proteins were qualified for analysis of their abilities to stimulate T-cell responses  in vitro . Then, an  in vitro  gamma interferon (IFN-γ) assay was used to identify potential T-cell antigens from  B. abortus . In total, 7 individual proteins that stimulated strong IFN-γ responses in splenocytes from mice immunized with  B. abortus  live vaccine S19 were identified. The protective efficiencies of these 7 recombinant proteins were further evaluated. Mice given BAB1_1316 (CobB) or BAB1_1688 (AsnC) plus adjuvant could provide protection against virulent  B. abortus  infection, similarly with the known protective antigen Cu-Zn SOD and the license vaccine S19. In addition, CobB and AsnC could induce strong antibodies responses in BALB/c mice. Altogether, the present study showed that CobB or AsnC protein could be useful antigen candidates for the development of subunit vaccines against brucellosis with adequate immunogenicity and protection efficacy.

Immunization of Mice with Recombinant Protein CobB or AsnC Confers Protection against Brucella abortus Infection