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Choloylglycine hydrolase (CGH, E.C. 3.5.1.24) is a conjugated bile salt hydrolase that catalyses the hydrolysis of the amide bond in conjugated bile acids. Bile salt hydrolases are expressed by gastrointestinal bacteria, and they presumably decrease the toxicity of host's conjugated bile salts.  Brucella  species are the causative agents of brucellosis, a disease affecting livestock and humans. CGH confers  Brucella  the ability to deconjugate and resist the antimicrobial action of bile salts, contributing to the establishment of a successful infection through the oral route in mice. Additionally,  cgh -deletion mutant was also attenuated in intraperitoneally inoculated mice, which suggests that CGH may play a role during systemic infection other than hydrolyzing conjugated bile acids. To understand the role CGH plays in  B. abortus  virulence, we infected phagocytic and epithelial cells with a  cgh -deletion mutant ( Δcgh)  and found that it is defective in the internalization process. This defect along with the increased resistance of  Δcgh  to the antimicrobial action of polymyxin B, prompted an analysis of the cell envelope of this mutant. Two-dimensional electrophoretic profiles of  Δcgh  cell envelope-associated proteins showed an altered expression of Omp2b and different members of the Omp25/31 family. These results were confirmed by Western blot analysis with monoclonal antibodies. Altogether, the results indicate that  Brucella  CGH not only participates in deconjugation of bile salts but also affects overall membrane composition and host cell internalization.

Brucella abortus Choloylglycine Hydrolase Affects Cell Envelope Composition and Host Cell Internalization