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RNA interference is an efficient method to silence gene and protein expressions. Here, the class B scavenger receptor CD36 ( SRB ) mediated the uptake of exogenous dsRNAs in the induction of the RNAi responses in ticks. Unfed female  Haemaphysalis longicornis  ticks were injected with a single or a combination of  H. longicornis SRB  ( HlSRB ) dsRNA, vitellogenin-1 ( HlVg-1 ) dsRNA, and vitellogenin receptor ( HlVgR ) dsRNA. We found that specific and systemic silencing of the  HlSRB ,  HlVg-1 , and  HlVgR  genes was achieved in ticks injected with a single dsRNA of  HlSRB ,  HlVg-1 , and  HlVgR . In ticks injected first with  HlVg-1  or  HlVgR  dsRNA followed 96 hours later with  HlSRB  dsRNA ( HlVg-1/HlSRB  or  HlVgR/HlSRB ), gene silencing of  HlSRB  was achieved in addition to first knockdown in  HlVg-1  or  HlVgR , and prominent phenotypic changes were observed in engorgement, mortality, and hatchability, indicating that a systemic and specific double knockdown of target genes had been simultaneously attained in these ticks. However, in ticks injected with  HlSRB  dsRNA followed 96 hours later with  HlVg-1  or  HlVgR  dsRNAs, silencing of  HlSRB  was achieved, but no subsequent knockdown in  HlVgR  or  HlVg-1  was observed. The Westernblot and immunohistochemical examinations revealed that the endogenous HlSRB protein was fully abolished in midguts of ticks injected with  HlSRB/HlVg-1  dsRNAs but HlVg-1 was normally expressed in midguts, suggesting that  HlVg-1  dsRNA-mediated RNAi was fully inhibited by the first knockdown of  HlSRB . Similarly, the abolished localization of HlSRB protein was recognized in ovaries of ticks injected with  HlSRB/HlVgR , while normal localization of HlVgR was observed in ovaries, suggesting that the failure to knock-down  HlVgR  could be attributed to the first knockdown of  HlSRB . In summary, we demonstrated for the first time that SRB may not only mediate the effective knock-down of gene expression by RNAi but also play essential roles for systemic RNAi of ticks.

Scavenger Receptor Mediates Systemic RNA Interference in Ticks