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Phosphatidylserine (PS) exposure on the cell surface indicates apoptosis, but has also been related to evasion mechanisms of parasites, a concept known as apoptotic mimicry.  Toxoplasma gondii  mimics apoptotic cells by exposing PS, inducing secretion of TGF-beta1 by infected activated macrophages leading to degradation of inducible nitric oxide (NO) synthase, NO production inhibition and consequently persisting in these cells. Here PS +  and PS −  subpopulation of tachyzoites were separated and the entrance mechanism, growth and NO inhibition in murine macrophages, and mice survival and pathology were analyzed. Infection index in resident macrophages was similar for both PS subpopulations but lower when compared to the total  T. gondii  population. Growth in resident macrophages was higher for the total  T. gondii  population, intermediate for the PS +  and lower for the PS −  subpopulation. Production of NO by activated macrophages was inhibited after infection with the PS +  subpopulation and the total populations of tachyzoites. However, the PS −  subpopulation was not able to inhibit NO production. PS +  subpopulation invaded macrophages by active penetration as indicated by tight-fitting vacuoles, but the PS −  subpopulation entered macrophages by phagocytosis as suggested by loose-fitting vacuoles containing these tachyzoites. The entrance mechanism of both subpopulations was confirmed in a non-professional phagocytic cell line where only the PS +  tachyzoites were found inside these cells in tight-fitting vacuoles. Both subpopulations of  T. gondii  killed mice faster than the total population. Clear signs of inflammation and no tachyzoites were seen in the peritoneal cavity of mice infected with the PS −  subpopulation. Moreover, mice infected with the PS +  subpopulation had no sign of inflammation and the parasite burden was intense. These results show that PS +  and PS −  subpopulations of  T. gondii  are necessary for a successful toxoplasma infection indicating that both subpopulations are required to maintain the balance between inflammation and parasite growth.

Phosphatidylserine Exposure by Toxoplasma gondii Is Fundamental to Balance the Immune Response Granting Survival of the Parasite and of the Host