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Both Neisseria meningitidis  and the closely related bacterium Neisseria lactamica colonise human nasopharyngeal mucosal surface, but only  N. meningitidis  invades the bloodstream to cause potentially life-threatening meningitis and septicaemia. We have hypothesised that the two neisserial species differentially modulate host respiratory epithelial cell gene expression reflecting their disease potential. Confluent monolayers of 16HBE14 human bronchial epithelial cells were exposed to live and/or dead  N. meningitidis  (including capsule and pili mutants) and  N. lactamica , and their transcriptomes were compared using whole genome microarrays. Changes in expression of selected genes were subsequently validated using Q-RT-PCR and ELISAs. Live  N. meningitidis  and  N. lactamica  induced genes involved in host energy production processes suggesting that both bacterial species utilise host resources.  N. meningitidis  infection was associated with down-regulation of host defence genes.  N. lactamica , relative to  N. meningitidis , initiates up-regulation of proinflammatory genes. Bacterial secreted proteins alone induced some of the changes observed. The results suggest  N. meningitidis  and  N. lactamica  differentially regulate host respiratory epithelial cell gene expression through colonisation and/or protein secretion, and that this may contribute to subsequent clinical outcomes associated with these bacteria.

Genome Wide Expression Profiling Reveals Suppression of Host Defence Responses during Colonisation by Neisseria meningitides but not N. lactamica