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Background  Seven serine/threonine kinase genes have been predicted in unicellular cyanobacterium  Synechocystis  sp. PCC6803. SpkA and SpkB were shown to be required for cell motility and SpkE has no kinase activity. There is no report whether the other four STKs are involved in stress-mediated signaling in  Synechocystis  PCC6803.    Methodology/Principal Findings  In this paper, we examined differential expression of the other four serine/threonine kinases,  SpkC ,  SpkD ,  SpkF  and  SpkG , at seven different stress conditions. The transcriptional level was up-regulated of  spkG  and down-regulated of  spkC  under high salt stress condition. Two  spk  deletion mutants, ΔspkC and ΔspkG, were constructed and their growth characteristic were examined compared to the wild strain. The wild strain and ΔspkC mutant were not affected under high salt stress conditions. In contrast, growth of  spkG  mutant was completely impaired. To further confirm the function of  spkG , we also examined the effect of mutation of  spkG  on the expression of salt stress-inducible genes. We compared genome-wide patterns of transcription between wild-type  Synechocystis  sp. PCC6803 and cells with a mutation in the  SpkG  with DNA microarray analysis.    Conclusion  In this study, we first study the  spkG  gene as sensor of high salt signal. We consider that  SpkG  play essential roles in  Synechocystis  sp. for sensing the high salt signal directly, rather than mediating signals among other kinases. Our microarray experiment may help select relatively significant genes for further research on mechanisms of signal transduction of  Synechocystis  sp. PCC6803 under high salt stress.

Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803