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Bovine spongiform encephalopathy  (BSE) surveillance programs have been employed in numerous countries to monitor BSE prevalence and to protect animal and human health. Since 1999, the European Commission (EC) authorized the evaluation and approval of 20 molecular based tests for the rapid detection of the pathological prion protein (PrP sc ) in BSE infection. The diagnostic sensitivity, convenience, and speed of these tests have made molecular diagnostics the preferred method for BSE surveillance. The aim of this study was to determine the analytical sensitivity of 4 commercially available BSE rapid-test kits, including the  Prionics®-Check WESTERN , the  Prionics® Check-PrioSTRIP ™, the  BioRad® TeSeE ™ ELISA, and the  IDEXX® HerdChek ™ EIA. Performances of these tests were then compared to 2 confirmatory tests, including the  BioRad® TeSeE ™  Western Blot  and the modified  Scrapie Associated Fibrils  ( SAF ) /OIE Immunoblot . One 50% w/v homogenate was made from experimentally generated C-type BSE brain tissues in ddH 2 O. Homogenates were diluted through a background of BSE-negative brainstem homogenate. Masses of both positive and negative tissues in each dilution were calculated to maintain the appropriate tissue amounts for each test platform. Specific concentrated homogenization buffer was added accordingly to maintain the correct buffer condition for each test. ELISA-based tests were evaluated using their respective software/detection platforms. Blot-protocols were evaluated by manual measurements of blot signal density. Detection limitations were determined by fitted curves intersecting the manufacturers' positive/negative criteria. The confirmatory SAF Immunoblot displayed the highest analytical sensitivity, followed by the IDEXX®  HerdChek ™ EIA,  Bio-Rad® TeSeE ™  Western Blot , the  Bio-Rad® TeSeE ™ ELISA,  Prionics®-Check PrioSTRIP ™, and  Prionics®-Check WESTERN ™, respectively. Although the tests performed at different levels of sensitivity, the most sensitive and least sensitive of the rapid tests were separated by 2 logs in analytical sensitivity, meeting European performance requirements. All rapid tests appear suitable for targeted BSE surveillance programs, as implemented in Canada.

A Study on the Analytical Sensitivity of 6 BSE Tests Used by the Canadian BSE Reference Laboratory