English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In methicillin resistant  Staphylococcus aureus  (MRSA), the arginine catabolic mobile element (ACME) was initially described in USA300 (t008-ST8) where it is located downstream of the staphylococcal cassette chromosome  mec  (SCC mec ). A common health-care associated MRSA in Copenhagen, Denmark (t024-ST8) is clonally related to USA300 and is frequently PCR positive for the ACME specific  arc A-gene. This study is the first to describe an ACME element upstream of the SCC mec  in MRSA. By traditional SCC mec  typing schemes, the SCC mec  of t024-ST8 strain M1 carries SCC mec  IVa, but full sequencing of the cassette revealed that the entire J3 region had no homology to published SCC mec  IVa. Within the J3 region of M1 was a 1705 bp sequence only similar to a sequence in  S. haemolyticus  strain JCSC1435 and 2941 bps with no homology found in GenBank. In addition to the usual direct repeats (DR) at each extremity of SCC mec , M1 had two new DR between the  orf X gene and the J3 region of the SCC mec . The region between the  orf X DR (DR1) and DR2 contained the  ccr AB4 genes. An ACME II-like element was located between DR2 and DR3. The entire 26,468 bp sequence between DR1 and DR3 was highly similar to parts of the ACME composite island of  S. epidermidis  strain ATCC12228. Sequencing of an ACME negative t024-ST8 strain (M299) showed that DR1 and the sequence between DR1 and DR3 was missing. The finding of a mobile ACME II-like element inserted downstream of  orf X and upstream of SCC mec  indicates a novel recombination between staphylococcal species.

An Unexpected Location of the Arginine Catabolic Mobile Element (ACME) in a USA300-Related MRSA Strain