English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  MALDI-TOF mass spectrometry (MS) is a reliable method for bacteria identification. Some databases used for this purpose lack reference profiles for  Brucella  species, which is still an important pathogen in wide areas around the world. We report the creation of profiles for MALDI-TOF Biotyper 2.0 database (Bruker Daltonics, Germany) and their usefulness for identifying brucellae from culture plates and blood cultures.    Methodology/Principal Findings  We created MALDI Biotyper 2.0 profiles for type strains belonging to  B. melitensis  biotypes 1, 2 and 3;  B. abortus  biotypes 1, 2, 5 and 9;  B. suis ,  B. canis ,  B ceti  and  B. pinnipedialis . Then, 131 clinical isolates grown on plate cultures were used in triplicate to check identification. Identification at genus level was always correct, although in most cases the three replicates reported different identification at species level. Simulated blood cultures were performed with type strains belonging to the main human pathogenic species ( B. melitensis ,  B. abortus ,  B. suis  and  B. canis ), and studied by MALDI-TOF MS in triplicate. Identification at genus level was always correct.    Conclusions/Significance  MALDI-TOF MS is reliable for  Brucella  identification to the genus level from culture plates and directly from blood culture bottles.

Identification of Brucella by MALDI-TOF Mass Spectrometry. Fast and Reliable Identification from Agar Plates and Blood Cultures